uid=knbadmin,o=NCEAS,dc=ecoinformatics,dc=org all uid=pmeirelles,o=marinebiodiversity,dc=ecoinformatics,dc=org all public read Hokkaido University Tomoo Sawabe Hokkaido University sawabe@fish.hokudai.ac.jp Pedro Meirelles Tomoo Sawabe associatedParty 2016-04-18 Coral reefs are most productive marine ecosystems serving as diversity-hotspot of active microbiomes. Corals around Ishigaki, however, are at risk due to tremendous stressors including elevated seawater temperature and bleaching events, eutrophication and inflow of specific soil. In particular, elevating temperature alters abundances and structures of natural coral Vibrio populations. However, no information is currently available on how Vibrio diversity is fluctuated spatially and temporally and what kinds of environmental determinants are correlated to the diversity in Ishigaki coral reef ecosystems. Three year assessments of Vibrio diversity study using 685 isolates from the surrounding seawater of coral reefs at different five geographic-sites on the basis of pyrH gene phylogeny revealed that Ishigaki coral reef ecosystems are occupied with diversified vibrios (32 known Vibrio clusters: 22 described spp.) including 26 potent novel vibrios from 12 clusters. The most prominent species were V. hyugaensis, V. owensii and V. harveyi, following V. maritimus-V.variabillis, V. campbellii, V. coralliilyticus, and P. rosenbergii. The Vibrio diversity fluctuations were rather less not to be varied not only year by year but also site by site based on UniFrac and simple cluster analyses. The AdaptML mathematical model predicted 8 inferred habitats from Ishigaki coral reef seawater vibrios considering their spatiotemporal distributions founded by pyrH gene phylogeny. Correlation analyses between an abundance of each major Vibrio species and an environmental determinant revealed significant positive correlations between rising seawater temperature and the abundance of V. campbellii (r=0.62; P>0.05), but vice versa between that and V. owensii (r=-0.58; P>0.05) and the C6 group of V. hyugaensis (r=-0.62; P>0.05). As Ishigaki coral reef is a less polluted area by antibiotics, natural occurrence of antibiotics resistance was assessed in representative vibrios, which are potent pathogenic to marine animals, such as V. coralliilyticus, V. harveyi, and V. alginolyticus. Multi-drug resistance profile investigation of the representative potent coral pathogens of this study showed resistant to all the tested clinically used antibiotics including meropenem (carbapenem) and ampicillin. These knowledge could be important clues not only in the future actions of coral conservations but also for emerging antibiotics resistance. Obtain permission from data set owner(s) digital 2012 2015 Ishigaki Island, Okinawa, Japan 124.2178 124.2178 24.3483 24.3483 Tomoo Sawabe Hokkaido University sawabe@fish.hokudai.ac.jp Sequences_Vibrio_Ishigaki.zip Sequences_Vibrio_Ishigaki.zip 51731 95981222b0ff7dc0e01d7b4e858ed9cc98a0617e application/zip ecogrid://knb/knb.25.1 uid=knbadmin,o=NCEAS,dc=ecoinformatics,dc=org all uid=pmeirelles,o=marinebiodiversity,dc=ecoinformatics,dc=org all public read Other